Sequence Variation in GAL1 and GAL2 Genes in Khuzestan Local Chickens

Abstract

Beta defensins were small, cationic peptides that bind to microbial membranes and disrupt their integrity, thereby exerting antimicrobial effects. These peptides play an important role in innate immunity against microbial pathogens. The aim of the present study was to detect sequence variations, if any, in some beta-defensin gene (GAL1 and GaL2) sequences in Khuzestan local chickens. Blood samples were collected from 20 local chickens and the genomic DNA was isolated using the standard phenol chloroform method. To detect sequence variation, the interested regions of GAL1 and GAL2 were amplified by specific primers with polymerase chain reaction (PCR). Then, the PCR products were sequenced in both directions. Analysis of the GAL1 gene found many sequence variations in the promoter region. GAL2 gene sequencing determined various sequence variations in intronic and exonic regions. These results suggest the existence of numerous genetic variations in these genomic sequences. Further association studies may help the development of a PCR-based genotyping test to select parents with a better immunity.