Effect of BA and Ovule Developmental Stages on Embryo Rescue in Perlette Grape (Vitis Vinifera L.) Cultivar

Abstract

Production of seedless varieties is one of the major breeding goals in grape vine. Traditional methods result in low percentage of seedless progeny production. Today for producing high percentage of seedless progeny, embryo rescue are used. In this research, the effects of BA concentrations (0, 30, 60 and 100 mg L-1) and the stage of ovule development (25, 35, 45, 55 and 65 days after pollination) were assessed on the success rate of the embryo rescue in Perlette grape (Vitis vinifera L.). BA was sprayed 14 days before and 7days after the anthesis. The berries were harvested at 25, 35, 45, 55 and 65 days post pollination. Ovules were cultured  in Nitch and Nitch medium with 0.35 mg L-1gibberellic acid (GA3) and 1 mg L-1 indolacetic acid (IAA). Characters such as germination, collapsed, callus-formed and enlarged ovules were recorded. The results revealed the effect of BA pre-treating and ovule developmental stage on percentage of germinated embryos, collapsed, callus- formed and enlarged ovules. The highest germination percentage was observed in BA 100 mg L-1 at 65 days post pollination treatment. The highest percentage of the collapsed ovules was observed at 25 days after pollination in all treatments. The highest percentage of the callus formation was observed in the BA treatment of 30 mg L-1 at 55 days after pollination. The highest percentage of the enlarged ovules was observed in the BA treatment of 60 mg L-1 at 45 days after pollination.